RapidPro™ Gravity

$198.00

RapidPro™ Gravity Kit: Instrument-Free, High-Purity Protein Purification

Overview

The RapidPro™ Gravity Kit is a silica-based protein purification system optimized for high- to very-high-expressed proteins in E. coli. Designed for researchers seeking a fast, efficient, and hazard-free alternative to traditional affinity chromatography, this kit delivers >98% purity in just 30-45 minutes—without requiring specialized equipment.

Key Features & Benefits

  • Passive purification with gravity flow for easy sample handling

  • Silica-based resin eliminates nickel-based waste

  • L-Lysine-based elution for safer, eco-friendly workflows

Performance Breakdown

  • Efficiency & Speed → Purification in 30-45 minutes with >98% purity

  • Capacity & Scalability → Handles 400 µL to 8 mL of lysate per column

  • High Performance → Optimized for high- to very high-expressed proteins in E. coli

  • Ease of Use → Works via gravity flow, no centrifugation or special instruments required

  • Eco-Friendly & Safe → No hazardous nickel resin or imidazole, uses L-Lysine for elution

Workflow & Applications

  • Simple no instrument purification: Load → Bind → Wash → Elute

  • Ideal for novice to experienced researchers, preliminary studies, and quality control testing

The RapidPro™ Gravity Kit is an affordable, scalable, and environmentally responsible solution for modern protein purification.

Intended for research use only. Formerly known as HEP Purification Kit.

Safety Data Sheets

RapidPro™ Gravity Kit: Instrument-Free, High-Purity Protein Purification

Overview

The RapidPro™ Gravity Kit is a silica-based protein purification system optimized for high- to very-high-expressed proteins in E. coli. Designed for researchers seeking a fast, efficient, and hazard-free alternative to traditional affinity chromatography, this kit delivers >98% purity in just 30-45 minutes—without requiring specialized equipment.

Key Features & Benefits

  • Passive purification with gravity flow for easy sample handling

  • Silica-based resin eliminates nickel-based waste

  • L-Lysine-based elution for safer, eco-friendly workflows

Performance Breakdown

  • Efficiency & Speed → Purification in 30-45 minutes with >98% purity

  • Capacity & Scalability → Handles 400 µL to 8 mL of lysate per column

  • High Performance → Optimized for high- to very high-expressed proteins in E. coli

  • Ease of Use → Works via gravity flow, no centrifugation or special instruments required

  • Eco-Friendly & Safe → No hazardous nickel resin or imidazole, uses L-Lysine for elution

Workflow & Applications

  • Simple no instrument purification: Load → Bind → Wash → Elute

  • Ideal for novice to experienced researchers, preliminary studies, and quality control testing

The RapidPro™ Gravity Kit is an affordable, scalable, and environmentally responsible solution for modern protein purification.

Intended for research use only. Formerly known as HEP Purification Kit.

Safety Data Sheets

Kit Components

  1. 125 mL Proteios Lysis L1 Buffer

  2. 125 mL Proteios Wash W1 Buffer

  3. 125 mL Proteios Elution E2 Buffer

  4. Pre-packed dry 12 mL Gravity Columns (Qty 8)

The target protein of interest (POI) can be expressed with a Proteios Car9 tag at either the N-terminal or C-terminal position. Proteios Car9-tagged POI binds to the Proteios Column Resin and can be released with the addition of the Proteios Elution Buffer.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.