RapidPro™ FPLC 5 mL
RapidPro™ FPLC 5 mL Kit: Advanced, High-Purity Protein Purification
Utilizing the innovative Car9 affinity tag, this kit efficiently purifies proteins from low to high expression levels in E. coli, delivering purification results with an average purity > 90% in just 40 minutes.
Designed to accommodate up to 5 mL of lysate, the kit supports flexible sample volumes, making it suitable for both standard applications and high-demand experimental setups. The kit includes pre-packed FPLC columns with a high binding capacity of 40 mg/mL, ensuring optimal performance for a variety of protein purification needs.
Ease of Use and Reliability: The RapidPro™ FPLC columns are designed for stability under a range of conditions (pH 5.0-8.5) and are compatible with all commonly used aqueous buffers, reducing agents, and detergents. With a recommended flow rate of 5 mL/min, this kit will simplify your purification process, maintaining structural integrity and performance across multiple uses.
Eco-Friendly and Safe: By leveraging a system that reduces reliance on hazardous materials and minimizes plastic waste, the RapidPro™ FPLC 5 mL Kit represents a greener choice for protein purification. The columns are reusable up to five times, reducing both operational costs and environmental impact.
RapidPro™ FPLC 5 mL Kit: Advanced, High-Purity Protein Purification
Utilizing the innovative Car9 affinity tag, this kit efficiently purifies proteins from low to high expression levels in E. coli, delivering purification results with an average purity > 90% in just 40 minutes.
Designed to accommodate up to 5 mL of lysate, the kit supports flexible sample volumes, making it suitable for both standard applications and high-demand experimental setups. The kit includes pre-packed FPLC columns with a high binding capacity of 40 mg/mL, ensuring optimal performance for a variety of protein purification needs.
Ease of Use and Reliability: The RapidPro™ FPLC columns are designed for stability under a range of conditions (pH 5.0-8.5) and are compatible with all commonly used aqueous buffers, reducing agents, and detergents. With a recommended flow rate of 5 mL/min, this kit will simplify your purification process, maintaining structural integrity and performance across multiple uses.
Eco-Friendly and Safe: By leveraging a system that reduces reliance on hazardous materials and minimizes plastic waste, the RapidPro™ FPLC 5 mL Kit represents a greener choice for protein purification. The columns are reusable up to five times, reducing both operational costs and environmental impact.
RapidPro™ FPLC 5 mL Kit: Advanced, High-Purity Protein Purification
Utilizing the innovative Car9 affinity tag, this kit efficiently purifies proteins from low to high expression levels in E. coli, delivering purification results with an average purity > 90% in just 40 minutes.
Designed to accommodate up to 5 mL of lysate, the kit supports flexible sample volumes, making it suitable for both standard applications and high-demand experimental setups. The kit includes pre-packed FPLC columns with a high binding capacity of 40 mg/mL, ensuring optimal performance for a variety of protein purification needs.
Ease of Use and Reliability: The RapidPro™ FPLC columns are designed for stability under a range of conditions (pH 5.0-8.5) and are compatible with all commonly used aqueous buffers, reducing agents, and detergents. With a recommended flow rate of 5 mL/min, this kit will simplify your purification process, maintaining structural integrity and performance across multiple uses.
Eco-Friendly and Safe: By leveraging a system that reduces reliance on hazardous materials and minimizes plastic waste, the RapidPro™ FPLC 5 mL Kit represents a greener choice for protein purification. The columns are reusable up to five times, reducing both operational costs and environmental impact.
Kit Components
250 mL L1 Lysis Buffer
250 mL W2 Wash Buffer
250 mL E1 Elution Buffer
5 mL pre-packed FPLC Columns (Qty 2)
The target protein of interest (POI) can be expressed with a Proteios Car9 tag at either the N-terminal or C-terminal position. Proteios Car9-tagged POI binds to the Proteios Column Resin and can be released with the addition of the Proteios Elution Buffer.