RapidPro™ FPLC 5 mL
RapidPro™ FPLC 5 mL Kit: High-Capacity, Fast Protein Purification
Overview
The RapidPro™ FPLC 5 mL Kit is designed for high-yield, scalable protein purification, achieving >90% purity in just 40 minutes. With a 5 mL resin bed, this system supports larger lysate volumes while maintaining exceptional binding efficiency and reproducibility.
Key Features & Benefits
5 mL pre-packed column, ideal for mid-to-large scale workflows
Applicable for a range of flow rates, and moderate pressure
Reusable more than 10 times, reducing overall purification costs
Performance Breakdown
Efficiency & Speed → Purification in 30 minutes with >90% purity
Capacity & Scalability → 5 mL resin bed with 40 mg/mL binding capacity
High Performance → Designed for larger-scale protein purification workflows
Ease of Use → Compatible with FPLC (ÄKTA™) and syringe-based setups
Eco-Friendly & Safe → Nickel-free, L-Lysine-based elution, reusable more than 10 times
Workflow & Applications
Quick, high-capacity purification: Load →Bind → Wash → Elute
Intended for research use only.
RapidPro™ FPLC 5 mL Kit: High-Capacity, Fast Protein Purification
Overview
The RapidPro™ FPLC 5 mL Kit is designed for high-yield, scalable protein purification, achieving >90% purity in just 40 minutes. With a 5 mL resin bed, this system supports larger lysate volumes while maintaining exceptional binding efficiency and reproducibility.
Key Features & Benefits
5 mL pre-packed column, ideal for mid-to-large scale workflows
Applicable for a range of flow rates, and moderate pressure
Reusable more than 10 times, reducing overall purification costs
Performance Breakdown
Efficiency & Speed → Purification in 30 minutes with >90% purity
Capacity & Scalability → 5 mL resin bed with 40 mg/mL binding capacity
High Performance → Designed for larger-scale protein purification workflows
Ease of Use → Compatible with FPLC (ÄKTA™) and syringe-based setups
Eco-Friendly & Safe → Nickel-free, L-Lysine-based elution, reusable more than 10 times
Workflow & Applications
Quick, high-capacity purification: Load →Bind → Wash → Elute
Intended for research use only.
RapidPro™ FPLC 5 mL Kit: High-Capacity, Fast Protein Purification
Overview
The RapidPro™ FPLC 5 mL Kit is designed for high-yield, scalable protein purification, achieving >90% purity in just 40 minutes. With a 5 mL resin bed, this system supports larger lysate volumes while maintaining exceptional binding efficiency and reproducibility.
Key Features & Benefits
5 mL pre-packed column, ideal for mid-to-large scale workflows
Applicable for a range of flow rates, and moderate pressure
Reusable more than 10 times, reducing overall purification costs
Performance Breakdown
Efficiency & Speed → Purification in 30 minutes with >90% purity
Capacity & Scalability → 5 mL resin bed with 40 mg/mL binding capacity
High Performance → Designed for larger-scale protein purification workflows
Ease of Use → Compatible with FPLC (ÄKTA™) and syringe-based setups
Eco-Friendly & Safe → Nickel-free, L-Lysine-based elution, reusable more than 10 times
Workflow & Applications
Quick, high-capacity purification: Load →Bind → Wash → Elute
Intended for research use only.
Kit Components
250 mL L1 Lysis Buffer
250 mL W2 Wash Buffer
250 mL E1 Elution Buffer
5 mL pre-packed FPLC Columns (Qty 2)
The target protein of interest (POI) can be expressed with a Proteios Car9 tag at either the N-terminal or C-terminal position. Proteios Car9-tagged POI binds to the Proteios Column Resin and can be released with the addition of the Proteios Elution Buffer.