Transforming Protein Purification: The Innovation Behind the RapidPro™ Gravity Kit
Protein purification is the backbone of breakthroughs in biochemistry, structural biology, and therapeutic development. Yet, traditional methods often create bottlenecks, demanding multiple steps, costly reagents, and specialized equipment. Imagine streamlining this process without sacrificing precision or performance. At Proteios Technology, we’ve achieved just that with the RapidPro™ Gravity Kit, which leverages innovative Car9 tag technology to simplify and enhance protein purification through an accessible gravity flow system.
This groundbreaking innovation bridges the gap between simplicity and precision, making high-quality protein purification accessible to researchers across disciplines and experience levels.
The Innovation Explained
The RapidPro™ Gravity kit is powered by Car9 tag technology, a proprietary affinity tag system that binds proteins to unmodified silica-based matrices with micromolar-level affinity. Here’s how it works:
Specific Silica Binding: The Car9 dodecapeptide exhibits micromolar binding affinity to Proteios' silica-based resin. This interaction, driven by hydrogen bonding and electrostatic forces, ensures robust protein immobilization without the need for chemically derivatized surfaces.
Enhanced Protocols: Designed for simplicity, the gravity column eliminates the need for specialized equipment, saving time and effort by requiring no additional steps for protein capture post-purification.
Eco-Friendly Buffers: RapidPro™ buffers are free of toxic or hazardous ingredients, removing the need for hazardous waste collection. Say goodbye to imidazole—forever!
These attributes make the Car9 system uniquely suited for rapid, high-yield purification under a variety of conditions and for a large variety of difficult-to-purify proteins.
Key Features of RapidPro™ Gravity
Gravity-Driven Purification: Eliminates the need for pumps or specialized instrumentation. Simply load the sample and collect purified protein at the column outlet.
High Purity in Minutes: Achieves protein purities exceeding 90% in less than 30 minutes, even from crude lysates.
Scalability and Cost Efficiency: At a fraction of the cost of conventional methods (e.g., His-tag/Ni-NTA systems), the RapidPro™ Gravity kit is scalable from research to proteomic applications.
Scientific Applications of Car9 Technology
Our research has demonstrated the versatility of Car9 technology in various scenarios. When used in conjunction with Proteios' silica-based columns, Car9-tagged proteins—such as GFPmut2, mCherry, and maltose-binding protein (MBP)—can be purified directly from clarified lysates with purities of 80–90%.
Structural Biology: Enables the rapid purification of proteins for crystallography and NMR studies.
Enzyme Engineering: Supports high-throughput screening of engineered biocatalysts.
Therapeutics and Drug Development: Facilitates the production of biologics for preclinical and early-stage research.
Functional Material Fabrication: Assists in creating hybrid protein-silica materials for biosensors and delivery systems.
Proteins are efficiently eluted using the optimized RapidPro™ elution buffer, reducing costs and streamlining workflows.
Commitment to Excellence
At Proteios Technology, we believe innovation should simplify science without compromising quality. The RapidPro™ Gravity Kit empowers researchers with a fast, efficient, and eco-friendly solution for protein purification. Curious to see the difference? Researchers in enzyme engineering and structural biology have already streamlined workflows with RapidPro™, achieving results in minutes rather than hours.
